MCP-1 in urine as biomarker of renal lupus in absence of cytokines, interferon-γ and growth factors

Barbado, Julia; Vega, Luisa; González-Gallego, Rocío; Jimeno, Antonio; De Lejarazu, Raúl Ortiz; Bermejo-Martin, Jesús F.

doi:10.1016/S2173-5743(10)70069-1

array:23 ["pii" => "S2173574310700691" "issn" => "21735743" "doi" => "10.1016/S2173-5743(10)70069-1" "estado" => "S300" "fechaPublicacion" => "2010-11-01" "aid" => "70069" "copyright" => "Sociedad Española de Reumatología and Colegio Mexicano de Reumatología" "copyrightAnyo" => "2010" "documento" => "article" "crossmark" => 0 "subdocumento" => "fla" "cita" => "Reumatol Clin. 2010;6:296-8" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 2602 "formatos" => array:3 [ "EPUB" => 47 "HTML" => 1387 "PDF" => 1168 ] ] "itemSiguiente" => array:18 [ "pii" => "S2173574310700708" "issn" => "21735743" "doi" => "10.1016/S2173-5743(10)70070-8" "estado" => "S300" "fechaPublicacion" => "2010-11-01" "aid" => "70070" "copyright" => "Sociedad Española de Reumatología and Colegio Mexicano de Reumatología" "documento" => "article" "crossmark" => 0 "subdocumento" => "fla" "cita" => "Reumatol Clin. 2010;6:299-302" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 2240 "formatos" => array:3 [ "EPUB" => 46 "HTML" => 1407 "PDF" => 787 ] ] "en" => array:10 [ "idiomaDefecto" => true "titulo" => "Frequency of systemic manifestations in patients with primary Sjögren's syndrome in Argentina" "tienePdf" => "en" "tieneTextoCompleto" => 0 "tieneResumen" => array:2 [ 0 => "en" 1 => "es" ] "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "299" "paginaFinal" => "302" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Frecuencia de manifestaciones sistémicas en pacientes con síndrome de Sjögren primario en Argentina" ] ] "contieneResumen" => array:2 [ "en" => true "es" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Federico Zazzetti, Mariano Adolfo Rivero, Damián Elvio Duartes Noé, Alberto Gallacher, Amalia Schiel, Marina Claudia Khoury, Hugo Armando Laborde, Juan Carlos Barreira" "autores" => array:8 [ 0 => array:2 [ "nombre" => "Federico" "apellidos" => "Zazzetti" ] 1 => array:2 [ "nombre" => "Mariano" "apellidos" => "Adolfo Rivero" ] 2 => array:2 [ "nombre" => "Damián Elvio" "apellidos" => "Duartes Noé" ] 3 => array:2 [ "nombre" => "Alberto" "apellidos" => "Gallacher" ] 4 => array:2 [ "nombre" => "Amalia" "apellidos" => "Schiel" ] 5 => array:2 [ "nombre" => "Marina" "apellidos" => "Claudia Khoury" ] 6 => array:2 [ "nombre" => "Hugo" "apellidos" => "Armando Laborde" ] 7 => array:2 [ "nombre" => "Juan" "apellidos" => "Carlos Barreira" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173574310700708?idApp=UINPBA00004M" "url" => "/21735743/0000000600000006/v1_201305061614/S2173574310700708/v1_201305061614/en/main.assets" ] "itemAnterior" => array:18 [ "pii" => "S217357431070068X" "issn" => "21735743" "doi" => "10.1016/S2173-5743(10)70068-X" "estado" => "S300" "fechaPublicacion" => "2010-11-01" "aid" => "70068" "copyright" => "Sociedad Española de Reumatología and Colegio Mexicano de Reumatología" "documento" => "article" "crossmark" => 0 "subdocumento" => "fla" "cita" => "Reumatol Clin. 2010;6:292-5" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 2665 "formatos" => array:3 [ "EPUB" => 51 "HTML" => 1878 "PDF" => 736 ] ] "en" => array:10 [ "idiomaDefecto" => true "titulo" => "Primary Sjögren's syndrome: Expression of NF-κB in minor salivary glands" "tienePdf" => "en" "tieneTextoCompleto" => 0 "tieneResumen" => array:2 [ 0 => "en" 1 => "es" ] "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "292" "paginaFinal" => "295" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Síndrome de Sjögren primario: expresión del factor NF-κB en glándula salival menor" ] ] "contieneResumen" => array:2 [ "en" => true "es" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Liliana Villalon, Marta Mamani, Felix E. Romanini, Antonio Catalan Pellet, Alejandro Berra" "autores" => array:5 [ 0 => array:2 [ "nombre" => "Liliana" "apellidos" => "Villalon" ] 1 => array:2 [ "nombre" => "Marta" "apellidos" => "Mamani" ] 2 => array:2 [ "nombre" => "Felix E." "apellidos" => "Romanini" ] 3 => array:2 [ "nombre" => "Antonio" "apellidos" => "Catalan Pellet" ] 4 => array:2 [ "nombre" => "Alejandro" "apellidos" => "Berra" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S217357431070068X?idApp=UINPBA00004M" "url" => "/21735743/0000000600000006/v1_201305061614/S217357431070068X/v1_201305061614/en/main.assets" ] "en" => array:14 [ "idiomaDefecto" => true "titulo" => "MCP-1 in urine as biomarker of renal lupus in absence of cytokines, interferon-γ and growth factors" "tieneTextoCompleto" => 0 "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "296" "paginaFinal" => "298" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Julia Barbado, Luisa Vega, Rocío González-Gallego, Antonio Jimeno, Raúl Ortiz De Lejarazu, Jesús F. Bermejo-Martin" "autores" => array:6 [ 0 => array:3 [ "nombre" => "Julia" "apellidos" => "Barbado" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "a" "identificador" => "aff0005" ] ] ] 1 => array:3 [ "nombre" => "Luisa" "apellidos" => "Vega" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "a" "identificador" => "aff0005" ] ] ] 2 => array:3 [ "nombre" => "Rocío" "apellidos" => "González-Gallego" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "a" "identificador" => "aff0005" ] ] ] 3 => array:3 [ "nombre" => "Antonio" "apellidos" => "Jimeno" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "a" "identificador" => "aff0005" ] ] ] 4 => array:3 [ "nombre" => "Raúl Ortiz" "apellidos" => "De Lejarazu" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "b" "identificador" => "aff0010" ] ] ] 5 => array:4 [ "nombre" => "Jesús F." "apellidos" => "Bermejo-Martin" "email" => array:1 [ 0 => "jbermejo@hcuv.sacyl.es" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "b" "identificador" => "aff0010" ] 1 => array:2 [ "etiqueta" => "¿" "identificador" => "cor0005" ] ] ] ] "afiliaciones" => array:2 [ 0 => array:3 [ "entidad" => "Unidad de Enfermedades Autoinmunes, Servicio de Medicina Interna, Hospital Clínico Universitario, Valladolid, Spain" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Unidad de Investigación en Infección e Inmunidad, Servicio de Microbiología e Inmunología, Hospital Clínico Universitario, IECSCYL, Valladolid, Spain" "etiqueta" => "b" "identificador" => "aff0010" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "MCP-1 en orina como biomarcador de lupus renal en ausencia de citoquinas, interferón-γ y factores de crecimiento" ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2009-07-15" "fechaAceptado" => "2009-09-29" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec112563" "palabras" => array:3 [ 0 => "Renal lupus" 1 => "Urine" 2 => "Immune mediators" ] ] ] "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec112562" "palabras" => array:3 [ 0 => "Lupus renal" 1 => "Orina" 2 => "Mediadores inmunológicos" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:2 [ "titulo" => "Abstract" "resumen" => "ObjectiveTo characterize 17 immunological markers in the Urine of patients with SLE. IntroductionLupus nephritis is an inflammatory disease affecting the renal parenchyma. Cytokines and chemokines are key immune mediators that have been related with the pathogenesis of the disease. Obtaining non invasive prognosis markers is a highly desirable objective in order to improve the clinical management of these patients. Patients and methodsIn this study we profiled 17 immune mediators (Th1, Th2, Th17 cytokines, chemokines and growth factors) in the urine of 25 patients with systemic lupus erythematosus with active renal disease by using a Biorad© 17-plex kit on a Luminex© platform. A group of healthy volunteers of similar age and comparable sex distribution was recruited as control (n=10). ResultsResults evidenced that the only detectable mediators in urine were IL-8, MCP-1 and MIP-1b. When levels of these mediators were compared between patients and controls, significantly higher levels of MCP-1 were observed in the urine of the patients. MCP-1 levels in urine correlated positively with the SLEDAI score in a significant way and negatively with plasma levels of complement C4. ConclusionsOur results reinforce the role of MCP-1 in urine as biomarker of disease activity in renal lupus, excluding the detection of other soluble immune mediators such as Th1, Th2, Th17 cytokines and growth factors as suitable markers in this non invasive sample." ] "es" => array:2 [ "titulo" => "Resumen" "resumen" => "ObjetivoPerfilar 17 medidores inmunológicos en la orina de pacientes con LES. Introducción: La nefritis lúpica es una enfermedad inflamatoria que afecta al parénquima renal. Citoquinas y quimiocinas son los mediadores inmunes dominantes relacionados con la patogénesis de la enfermedad. La obtención de marcadores de pronóstico no invasivos es un objetivo sumamente deseable para mejorar el manejo clínico de estos pacientes. Pacientes y métodosEn este estudio nosotros perfilamos 17 mediadores inmunológicos (citoquinas Th1, Th2, Th17, quimiocinas y factores de crecimiento) en la orina de 25 pacientes con lupus y enfermedad renal activa usando un kit Biorad© 17-plex en plataforma Luminex©. Como grupo control se seleccionaron (n=10) voluntarios sanos con similar edad y sexo que los casos. ResultadosLos resultados evidenciaron que los únicos mediadores perceptibles en orina eran IL-8, MCP-1 y MIP-1b. Cuando los niveles de estos mediadores fueron comparados entre los pacientes y los controles, MCP-1 en la orina de los casos fue el único que aumentó de forma significativa (p<0,05) con respecto a los controles. Estos niveles de MCP-1 en orina se correlacionaron de forma positiva con la puntuación de SLEDAI y de forma negativa con los niveles en plasma de la proteína C4 del sistema de complemento. ConclusionesNuestros resultados refuerzan el papel de MCP-1 en orina como biomarcador no invasivo de actividad de enfermedad en el lupus renal, no evidenciando la detección de otros mediadores inmunológicos solubles, como son las citoquinas Th1, Th2, Th17 y factores de crecimiento." ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:13 [ 0 => array:3 [ "identificador" => "bib1" "etiqueta" => "1." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Urine osteoprotegerin and monocyte chemoattractant protein-1 in lupus nephritis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "A. Kiani" 1 => "K. Johnson" 2 => "C. Chen" 3 => "E. Diehl" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:2 [ "tituloSerie" => "J Rheumatol" "fecha" => "2009" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib2" "etiqueta" => "2." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Chemoattractant mechanism of Th1 cells in class III and IV lupus nephritis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "S. Morimoto" 1 => "Y. Tokano" 2 => "S. Nakano" 3 => "T. Watanabe" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1080/08916930802438790" "Revista" => array:6 [ "tituloSerie" => "Autoimmunity" "fecha" => "2009" "volumen" => "42" "paginaInicial" => "143" "paginaFinal" => "149" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19021013" "web" => "Medline" ] ] ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib3" "etiqueta" => "3." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "M. Tucci" 1 => "C. Quatraro" 2 => "L. Lombardi" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1002/art.23186" "Revista" => array:7 [ "tituloSerie" => "Arthritis Rheum" "fecha" => "2008" "volumen" => "58" "paginaInicial" => "251" "paginaFinal" => "262" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18163476" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S0015028201028588" "estado" => "S300" "issn" => "00150282" ] ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib4" "etiqueta" => "4." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Interleukin-20 targets renal mesangial cells and is associated with lupus nephritis" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "H.H. Li" 1 => "H.H. Cheng" 2 => "K.H. Sun" 3 => "C.C. Wei" 4 => "C.F. Li" 5 => "W.C. Chen" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.clim.2008.07.006" "Revista" => array:6 [ "tituloSerie" => "Clin Immunol" "fecha" => "2008" "volumen" => "129" "paginaInicial" => "277" "paginaFinal" => "285" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18771958" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib5" "etiqueta" => "5." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Glomerular expression of monocyte chemoattractant protein-1 is predictive of poor renal prognosis in paediatric lupus nephritis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "D.M. Stephen" 1 => "J.W. Sue" 2 => "T. Kjell" 3 => "J.S. Neil" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1093/ndt/gfn270" "Revista" => array:6 [ "tituloSerie" => "Nephrol Dial Transplant" "fecha" => "2008" "volumen" => "23" "paginaInicial" => "3521" "paginaFinal" => "3526" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18495743" "web" => "Medline" ] ] ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bib6" "etiqueta" => "6." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Correlative insights into immunoexpression of monocyte chemoattractant protein-1, transforming growth factor beta-1 and CD68+ cells in lupus nephritis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "M. Wagrowska-Danilewicz" 1 => "O. Stasikowska" 2 => "M. Danilewicz" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Pol J Pathol" "fecha" => "2005" "volumen" => "56" "paginaInicial" => "115" "paginaFinal" => "120" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16334978" "web" => "Medline" ] ] ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bib7" "etiqueta" => "7." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:1 [ "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "S. Tian" 1 => "J. Li" 2 => "L. Wang" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Urinary levels of RANTES and M-CSF are predictors of lupus nephritis flare" "fecha" => "2007" "volumen" => "56" "paginaInicial" => "304" "paginaFinal" => "310" ] ] ] ] ] ] 7 => array:3 [ "identificador" => "bib8" "etiqueta" => "8." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Systemic lupus erythematosus" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "A. Rahman" 1 => "D.A. Isenberg" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1056/NEJMra071297" "Revista" => array:6 [ "tituloSerie" => "N Engl J Med" "fecha" => "2008" "volumen" => "358" "paginaInicial" => "929" "paginaFinal" => "939" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18305268" "web" => "Medline" ] ] ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib9" "etiqueta" => "9." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Urine chemokines: Biomarkers of human lupus nephritis?" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "G.M. Ferri" 1 => "A. Gigante" 2 => "F. Ferri" 3 => "D. Amoroso" 4 => "E. Zardi" 5 => "A. Papa" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Eur Rev Med Pharmacol Sci" "fecha" => "2007" "volumen" => "11" "paginaInicial" => "171" "paginaFinal" => "178" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17970233" "web" => "Medline" ] ] ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib10" "etiqueta" => "10." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Glomerular expression of monocyte chemoattractant protein-1 is predictive of poor renal prognosis in pediatric lupus nephritis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "S.D. Marks" 1 => "S.J. Williams" 2 => "K. Tullus" 3 => "N.J. Sebire" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1093/ndt/gfn270" "Revista" => array:6 [ "tituloSerie" => "Nephrol Dial Transplant" "fecha" => "2008" "volumen" => "23" "paginaInicial" => "3521" "paginaFinal" => "3526" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18495743" "web" => "Medline" ] ] ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bib11" "etiqueta" => "11." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Urine chemokines as biomarkers of human systemic lupus erythematosus activity" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "B.H. Rovin" 1 => "H. Song" 2 => "D.J. Birmingham" 3 => "L.A. Hebert" 4 => "C.Y. Yu" 5 => "H.N. Nagaraja" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1681/ASN.2004080658" "Revista" => array:6 [ "tituloSerie" => "J Am Soc Nephrol" "fecha" => "2005" "volumen" => "16" "paginaInicial" => "467" "paginaFinal" => "473" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15601744" "web" => "Medline" ] ] ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bib12" "etiqueta" => "12." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The 1982 revised criteria for the classification of systemic lupus erythematosus" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "E.M. Tan" 1 => "A.S. Cohen" 2 => "J.F. Fries" 3 => "A.T. Masi" 4 => "D.J. Mcshane" 5 => "N.F. Rothfield" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:7 [ "tituloSerie" => "Arthritis Rheum" "fecha" => "1982" "volumen" => "25" "paginaInicial" => "1271" "paginaFinal" => "1277" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7138600" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S0015028204006843" "estado" => "S300" "issn" => "00150282" ] ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bib13" "etiqueta" => "13." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Systemic Lupus Erythematosus Disease Activity Index 2000" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "D.D. Gladman" 1 => "D. Ibanez" 2 => "M.B. Urowitz" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "J Rheumatol" "fecha" => "2002" "volumen" => "29" "paginaInicial" => "288" "paginaFinal" => "291" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11838846" "web" => "Medline" ] ] ] ] ] ] ] ] ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/21735743/0000000600000006/v1_201305061614/S2173574310700691/v1_201305061614/en/main.assets" "Apartado" => array:4 [ "identificador" => "8426" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Brief original" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/21735743/0000000600000006/v1_201305061614/S2173574310700691/v1_201305061614/en/main.pdf?idApp=UINPBA00004M&text.app=https://reumatologiaclinica.org/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173574310700691?idApp=UINPBA00004M"]

Article information

Statistics

Abstract

Objective

To characterize 17 immunological markers in the Urine of patients with SLE.

Introduction

Lupus nephritis is an inflammatory disease affecting the renal parenchyma. Cytokines and chemokines are key immune mediators that have been related with the pathogenesis of the disease. Obtaining non invasive prognosis markers is a highly desirable objective in order to improve the clinical management of these patients.

Patients and methods

In this study we profiled 17 immune mediators (Th1, Th2, Th17 cytokines, chemokines and growth factors) in the urine of 25 patients with systemic lupus erythematosus with active renal disease by using a Biorad© 17-plex kit on a Luminex© platform. A group of healthy volunteers of similar age and comparable sex distribution was recruited as control (n=10).

Results

Results evidenced that the only detectable mediators in urine were IL-8, MCP-1 and MIP-1b. When levels of these mediators were compared between patients and controls, significantly higher levels of MCP-1 were observed in the urine of the patients. MCP-1 levels in urine correlated positively with the SLEDAI score in a significant way and negatively with plasma levels of complement C4.

Conclusions

Our results reinforce the role of MCP-1 in urine as biomarker of disease activity in renal lupus, excluding the detection of other soluble immune mediators such as Th1, Th2, Th17 cytokines and growth factors as suitable markers in this non invasive sample.

Keywords:

Renal lupus

Urine

Immune mediators

Resumen

Objetivo

Perfilar 17 medidores inmunológicos en la orina de pacientes con LES. Introducción: La nefritis lúpica es una enfermedad inflamatoria que afecta al parénquima renal. Citoquinas y quimiocinas son los mediadores inmunes dominantes relacionados con la patogénesis de la enfermedad. La obtención de marcadores de pronóstico no invasivos es un objetivo sumamente deseable para mejorar el manejo clínico de estos pacientes.

Pacientes y métodos

En este estudio nosotros perfilamos 17 mediadores inmunológicos (citoquinas Th1, Th2, Th17, quimiocinas y factores de crecimiento) en la orina de 25 pacientes con lupus y enfermedad renal activa usando un kit Biorad© 17-plex en plataforma Luminex©. Como grupo control se seleccionaron (n=10) voluntarios sanos con similar edad y sexo que los casos.

Resultados

Los resultados evidenciaron que los únicos mediadores perceptibles en orina eran IL-8, MCP-1 y MIP-1b. Cuando los niveles de estos mediadores fueron comparados entre los pacientes y los controles, MCP-1 en la orina de los casos fue el único que aumentó de forma significativa (p<0,05) con respecto a los controles. Estos niveles de MCP-1 en orina se correlacionaron de forma positiva con la puntuación de SLEDAI y de forma negativa con los niveles en plasma de la proteína C4 del sistema de complemento.

Conclusiones

Nuestros resultados refuerzan el papel de MCP-1 en orina como biomarcador no invasivo de actividad de enfermedad en el lupus renal, no evidenciando la detección de otros mediadores inmunológicos solubles, como son las citoquinas Th1, Th2, Th17 y factores de crecimiento.

Palabras clave:

Lupus renal

Orina

Mediadores inmunológicos

Full text is only aviable in PDF

References

[1.]

A. Kiani, K. Johnson, C. Chen, E. Diehl.

Urine osteoprotegerin and monocyte chemoattractant protein-1 in lupus nephritis.

J Rheumatol, (2009),

[2.]

S. Morimoto, Y. Tokano, S. Nakano, T. Watanabe.

Chemoattractant mechanism of Th1 cells in class III and IV lupus nephritis.

Autoimmunity, 42 (2009), pp. 143-149

http://dx.doi.org/10.1080/08916930802438790 | Medline

[3.]

M. Tucci, C. Quatraro, L. Lombardi.

Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18.

Arthritis Rheum, 58 (2008), pp. 251-262

http://dx.doi.org/10.1002/art.23186 | Medline

[4.]

H.H. Li, H.H. Cheng, K.H. Sun, C.C. Wei, C.F. Li, W.C. Chen, et al.

Interleukin-20 targets renal mesangial cells and is associated with lupus nephritis.

Clin Immunol, 129 (2008), pp. 277-285

http://dx.doi.org/10.1016/j.clim.2008.07.006 | Medline

[5.]

D.M. Stephen, J.W. Sue, T. Kjell, J.S. Neil.

Glomerular expression of monocyte chemoattractant protein-1 is predictive of poor renal prognosis in paediatric lupus nephritis.

Nephrol Dial Transplant, 23 (2008), pp. 3521-3526

http://dx.doi.org/10.1093/ndt/gfn270 | Medline

[6.]

M. Wagrowska-Danilewicz, O. Stasikowska, M. Danilewicz.

Correlative insights into immunoexpression of monocyte chemoattractant protein-1, transforming growth factor beta-1 and CD68+ cells in lupus nephritis.

Pol J Pathol, 56 (2005), pp. 115-120

Medline

[7.]

S. Tian, J. Li, L. Wang.

Urinary levels of RANTES and M-CSF are predictors of lupus nephritis flare, 56 (2007), pp. 304-310

[8.]

A. Rahman, D.A. Isenberg.

Systemic lupus erythematosus.

N Engl J Med, 358 (2008), pp. 929-939

http://dx.doi.org/10.1056/NEJMra071297 | Medline

[9.]

G.M. Ferri, A. Gigante, F. Ferri, D. Amoroso, E. Zardi, A. Papa, et al.