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Vol. 18. Issue 3.
Pages 147-149 (March 2022)
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Vol. 18. Issue 3.
Pages 147-149 (March 2022)
Original Article
Are the specific and nonspecific ANA staining patterns of Behçet's Disease patients important?
¿Son importantes los patrones de tinción ANA específicos y no específicos para los pacientes con enfermedad de Behçet?
Fulya Ilhana, Rahime Aksoyb,
Corresponding author

Corresponding author.
, Hüseyin Tutkakc
a Department of Immunology, Faculty of Medicine, Elazığ University, Elaziğ, Turkey
b Department of Hematology, Faculty of Medicine, Ankara University, Ankara, Turkey
c Department of Immunology and Allergy Diseases, Faculty of Medicine, Ankara University, Ankara, Turkey
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Tables (1)
Table 1. Dyeing patterns detected in patients and controls at 1/100 dilution.

The autoinflammatory character of Behçet's Disease has led researchers to investigate the role of autoantibodies. However, no significant positive result has been reported for autoantibody tests for the disease.


To investigate the specific and nonspecific staining patterns of Behçet's Disease (BD) patients.


140 patients (87 females, 53 males) with an average of 41.9±3 years who were being followed up for Behçet's Disease, and a control group consisting of a total of 736 (464 females, 272 males) healthy volunteers made up of blood donors without any disease whose average age was 50.2±4 years were included in the study. Peripheral venous blood was collected from the patients and the sera were separated. Patient sera were studied by indirect immunofluorescence antibody test (IFA) at a dilution of 1/40 and 1/100.


A total of 140 (87 females, 53 males) Behçet's Disease patients and 736 (464 females, 272 males) healthy controls were examined. The rate of ANA positivity was 11.6% in the control group and 10.7% in the Behçet's Disease group. In general, no difference was detected between the patients and the healthy controls in terms of autoantibody positivity (p>0.05). However, when examined in terms of patterns, the low detection of DFS70 and the observation of centriole staining type patterns in Behçet's Disease patients was noteworthy (p<0.05).


Autoantibody tests, which hold an important place in classic autoimmune diseases, are not necessary for Behçet's patients, but they should be examined in terms of nonspecific patterns.

ANA staining patterns
Behçet's Disease

El carácter autoinflamatorio de la enfermedad de Behçet (EB) ha llevado a los investigadores a estudiar el rol de los autoanticuerpos. Sin embargo, no se ha reportado un resultado positivo significativo para las pruebas de autoanticuerpos.


Investigar los patrones de tinción específicos y no específicos de los pacientes con EB.


Se incluyó en el estudio a 140 pacientes (87 mujeres y 53 varones) con una edad media de 41,9±3 años con seguimiento por EB, y un grupo control que incluyó a un total de 736 voluntarios sanos (464 mujeres y 272 varones) integrados por donantes de sangre sin enfermedad alguna, con una edad media de 50,2±4 años. Se extrajo sangre de vena periférica a todos los pacientes, separándose el suero, que se estudió mediante el test de anticuerpos por inmunofluorescencia directa (IFA) a un factor de dilución de 1/40 y 1/100.


Se examinó a un total de 140 pacientes (87 mujeres y 53 varones) con EB y 736 controles sanos (464 mujeres y 272 varones). La tasa de positividad de ANA fue del 11,6% en el grupo control y del 10,7% en el grupo de EB. En general no se detectó diferencia entre los pacientes y los controles sanos en términos de positividad de autoanticuerpos (p>0,05). Sin embargo, al realizarse el examen en términos de patrones, fue destacable la baja detección de DFS70 y la observación de los patrones tipo tinción de centriolos en los pacientes con EB (p<0,05).


Los test de autoanticuerpos, que ocupan una posición importante en las enfermedades autoinmunes clásicas, no son necesarios para los pacientes con EB, aunque deberían examinarse en términos de patrones no específicos.

Palabras clave:
Patrones de tinción del método ANA
Enfermedad de Behçet


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