Información de la revista
Vol. 2. Núm. 6.
Páginas 289-293 (noviembre - diciembre 2006)
Vol. 2. Núm. 6.
Páginas 289-293 (noviembre - diciembre 2006)
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Patrón de citocinas séricas en pacientes con artritis reumatoide de acuerdo a su reactividad al PPD
Pattern of serum cytokines in patients with rheumatoid artritis according to PPD reactivity
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Darío Ponce de León Pandolfi
Autor para correspondencia
edacvas@terra.com.pe

Correspondencia: Dr. D. Ponce de León Pandolfi. Hospital Nacional Guillermo Almenara Irigoyen. Red Asistencial Almenara. EsSalud. Lima. Avda. Grau, 800. Lima 13. Perú.
, César Pastor Asurza, Yasmina Beraun, Eduardo Acevedo-Vásquez, Alfredo Sánchez-Torres, José Alfaro Lozano, Risto Perich Campos, Mariano Cucho Venegas, César Gutiérrez Villafuerte, César Sánchez Schwartz
Servicio de Reumatología. Hospital Nacional Guillermo Almenara Irigoyen-Red Asistencial Almenara. EsSalud. Lima. Perú
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Introducción

En un reciente estudio, se demostró una alta reactividad negativa al PPD o tuberculina en pacientes con artritis reumatoide (AR), (70%) comparado con controles (30%). Para determinar si esta alta reactividad negativa al PPD está asociada con un determinado patrón de citocinas, se compararon las concentraciones séricas de interleucina (IL)-2, IL-4, IL-6, IL-10, factor de necrosis tumoral (TNF)-α e interferón (IFN)-γ en pacientes con AR con reactividad positiva y negativa al PPD. Se analizó también la correlación entre las citocinas y la actividad de la AR.

Material y métodos

Se estudiaron 40 pacientes con AR y 21 individuos sanos. Se consideró reactividad positiva al PPD a una induración ≥ 5mm y reactividad negativa cuando es < 5 mm. La actividad de la AR se determinó según el DAS28. Las citocinas se determinaron por citometría de flujo utilizando el Kit Multiplex Cytometric Bead Array.

Resultados

De los pacientes con AR, 27 (67,5%) presentaron reactividad negativa al PPD y 13 (32,5%) reactividad positiva al PPD, similares en edad, sexo femenino y enfermedad activa. No se encontraron diferencias significativas en las citocinas entre los grupos con PPD positivo y PPD negativo. El IFN-γ (r = 0,433; p = 0,005) y la IL-6 (r = 0,325; p = 0,041) fueron las únicas que mostraron correlación positiva con la actividad de la enfermedad.

Conclusiones

No parece que haya diferencias en el patrón de citocinas séricas en los pacientes con reactividad negativa y positiva al PPD.

Key words:
Rheumatoid arthritis (RA)
PPD
Cytokines
Palabras clave:
Artritis reumatoide (AR)
PPD
Citocinas
Introduction

We demonstrated, in a recently published study, far more PPD negative reactivity among patients who had RA (70%) than among controls (30%). To evaluate the hypothesis that different response to PPD in RA patients is associated with different profiles of serum cytokines, we compared the serum levels of IL-2, IL-4, IL-6, IL-10, TNF alpha and IFN gamma from PPD negative and PPD positive RA patients. We also evaluated any correlations between serum cytokines and RA activity.

Material and methods

Forty RA patients and 21 controls were enrolled. Those with an induration < 5mm were considered as negative and those with ≥ 5mm as positive PPD. Disease activity was calculated using DAS28. Plasma levels of cytokines were determined using the multiplex BD TM Cytometric Bead Array Kit Assay.

Results

Of the RA patients, 27 (67.5%) had negative reaction to PPD and 13 (32.5%) a positive reaction to PPD. There was no statistical difference in sex profile, age or activity index between both negative and positive PPD RA patients. There was no significant difference in all the cytokines measured between PPD positive and PPD negative RA patients. Index activity show a positive correlation with IFN gamma (r = 0.433; p = 0.005) and IL-6 (r = 0.325;

p = 0.041) in RA patients.

Conclusions

Positive and negative tuberculin RA patients seem to show a similar cytokine serum profile.

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Copyright © 2006. Elsevier España S.L. Barcelona
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