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Vol. 1. Núm. 4.
Páginas 187-192 (Noviembre - Diciembre 2005)
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Vol. 1. Núm. 4.
Páginas 187-192 (Noviembre - Diciembre 2005)
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Búsqueda de nuevos autoantígenos en el síndrome de Sjögren
The search for new autoantigens in Sjögren's syndrome
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I. Ferraz-Amaroa,??
Autor para correspondencia
iferraz@comtf.es

Correspondencia: Dr. I. Ferraz-Amaro. Servicio de Reumatología. Hospital Universitario de Canarias. Ofra, s/n. La Cuesta. 38320 Santa Cruz de Tenerife. España.
, I. Cozar-Castellanob, M.F. Arteagab, M.V. Machargob, E. Acostab, J. Ávilab, S. Bustabada, E. Trujilloa, T. Gonzáleza, P. Martín-Vasallob
a Servicio de Reumatología. Hospital Universitario de Canarias. Santa Cruz de Tenerife. España
b Laboratorio de Biología del Desarrollo. Departamento de Biología Molecular. Universidad de La Laguna. Santa Cruz de Tenerife. España
Información del artículo
Resumen
Bibliografía
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Estadísticas
Objetivo

Determinar la existencia de nuevos autoantígenos en el síndrome de Sjögren (SS) así como estudiar la prevalencia de éstos en pacientes y población sana.

Material y métodos

Se procedió a realizar un muestreo con el suero de una enferma afectada de SS mediante la utilización de una genoteca de cerebro humano (técnica SEREX). Se determinó que este suero expresaba autoantígenos ya conocidos y proteínas no descritas previamente, y también se confirmó la presencia de una proteína desconocida hasta ahora. De entre ellas, se seleccionó a esta última (hIscA) y a la proteína Tau (hallada en el muestreo) para ser transformadas en sendos plásmidos de expresión para conseguir su síntesis recombinante.

Resultados

Mediante técnica de inmunotransferencia se testó la existencia de anticuerpos anti-Tau y anti-hIscA en 19 pacientes y 20 sujetos sanos. No se encontró diferencia estadísticamente significativa entre pacientes y controles en la expresión de anticuerpos anti-Tau y se halló que los pacientes expresaban, de forma significativa,valores inferiores de anticuerpos anti-hIscA.

Conclusión

Se ha identificado a las proteínas Tau y hIscA como nuevos autoantígenos en el SS. Se ha hallado que los pacientes con SS expresan valores inferiores de anticuerpos anti-hIscA en comparación con controles y, aunque no se ha encontrado ninguna diferencia entre sanos y enfermos en relación con la presencia de anticuerpos anti-Tau, ésta es la primera vez en que anticuerpos contra esta proteína se han detectado en el SS.

Palabras clave:
Síndrome de Sjögren
Autoantígenos
Autoanticuerpos
Autoinmunidad
Objective

To identify new autoantigens related to Sjögren's syndrome and to determine their prevalence in patients and healthy individuals.

Material and methods

Serological sampling was performed in a patient with Sjögren's syndrome through the use of a human brain expression genotec (SEREX technique) to determine expression of known autoantigens and previously undescribed proteins. The presence of a previously unknown protein was found. Several proteins were obtained and two were selected to be studied (a human protein called Tau and an unknown protein described by our group and named hlscA). Both Tau and hIscA cDNA were transformed into an expression plasmid to obtain their recombinant proteins.

Results

Using a Western-blot technique we investigated the presence of anti-Tau and anti-hlscA autoantibodies in the sera of 19 patients with Sjögren's syndrome and in the sera of 20 controls. No statistically significant differences were found in the expression of anti-Tau antibodies between patients with Sjögren's syndrome and controls but values of anti-hlscA autoantibodies were significantly lower in patients with Sjögren's syndrome.

Conclusion

We identified Tau and hIscA proteins as new autoantigens in Sjögren's syndrome. Anti-hlscA antibody values were significantly lower in patients with Sjögren's syndrome than in healthy controls. Although no statistically significant differences in values of anti-Tau antibodies were found between Sjögren's syndrome patients and controls, this is the first time antibodies against this protein have been detected in Sjögren's syndrome.

Key words:
Sjögren's syndrome
Autoantigens
Autoantibodies
Autoimmunity
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Copyright © 2005. Elsevier España S.L. Barcelona
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